Job Description

Nona Biosciences is seeking a highly skilled and experienced scientist to join our team in Natick, MA. The successful candidate will play a critical role in establishing antibody discovery and production capabilities in our US lab. The main responsibilities of this role include designing, producing, purifying, and analyzing antibodies to support all projects. The ideal candidate should have hands-on experience with CHO and 293 cell culture and transfection techniques, as well as expertise in antibody purification.

Responsibilities

• Design and source DNA constructs used for antibody expression.
• Perform plasmid transfection in mammalian cells (CHO or HEK293) for antibody expression.
• Antibody or other biologics purification by the affinity chromatography.
• Characterize and quality control of purified antibodies using variety of analytical methods (SEC, SDS-PAGE, FACS etc.)
• Maintain a detailed and well-organized record of experiments in electronic notebook.
• Collaborate with other team members to support internal and external projects
• Help organize, maintain and supply the laboratory

Requirements

• BS./M.S. in biochemistry, molecular biology, protein chemistry or related fields.
• 2+ years of hands-on experience with mammalian cell culture and recombinant mammalian protein expression, such as CHO and HEK293 cells.
• Experienced with protein purification techniques, such as affinity chromatography and SEC.
• Knowledge with protein characterization and quality control techniques, such as protein concentration measurement, SDS-PAGE, Western blot, and ELISA.
• Excellent organization/planning skills to meet deadlines and handle multiple tasks simultaneously
• Diligent, self-motivated, independent and strong sense of responsibility
• Good interpersonal skills for building relationships across functions internally and externally

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